Purification:Article Title: Antioxidant and anti-inflammatory function of walnut green husk aqueous extract (WNGH-AE) on human hepatocellular carcinoma cells (HepG2) treated with t-BHP
Article Snippet: Finally, the IL-1β assay kit and TNF-α assay kit were sourced from Boster Bioengineering Co., Ltd. in Wuhan, China. .. The study utilized several instruments, including a double distilled deionized water system (laboratory purified water system, Shanghai Hitech Instrument Co., Ltd.), an electronic balance (PX224ENH, Germany OHAUS Instrument Co., Ltd.), a carbon dioxide incubator (HF90, Shanghai Heli Biopharmaceutical Holdings Co., Ltd.), an inverted biological microscope (BDS500, Chongqing Optec Instrument Co., Ltd.), an ELISA microplate analyzer (DNM-9602G, Beijing Perlove Co., Ltd.), a rotary evaporator (RE-2000A, Shanghai Hitech Instrument Co., Ltd.), an ultrasonic extractor (SB25-12DDT, Ningbo Xinzhi Biotechnology Co., Ltd.), and a freeze-drying instrument (SCIENTZ-10N, Ningbo Xinzhi Biotechnology Co., Ltd.), an ultra-high performance liquid chromatography (UPLC) (ACQUITY, Waters, Milford), an ACQUITY UPLC® HSS T3 column (2.1×150 mm, 1.8 μm) (Waters, Milford), an Thermo Q Exactive (Thermo Fisher Scientific). ..
Article Title: Antioxidant and anti-inflammatory function of walnut green husk aqueous extract (WNGH-AE) on human hepatocellular carcinoma cells (HepG2) treated with t-BHP.
Article Snippet: Finally, the IL-1β assay kit and TNF-α assay kit were sourced from Boster Bioengineering Co., Ltd. in Wuhan, China. .. The study utilized several instruments, including a double distilled deionized water system (laboratory purified water system, Shanghai Hitech Instrument Co., Ltd.), an electronic balance (PX224ENH, Germany OHAUS Instrument Co., Ltd.), a carbon dioxide incubator (HF90, Shanghai Heli Biopharmaceutical Holdings Co., Ltd.), an inverted biological microscope (BDS500, Chongqing Optec Instrument Co., Ltd.), an ELISA microplate analyzer (DNM-9602G, Beijing Perlove Co., Ltd.), a rotary evaporator (RE-2000A, Shanghai Hitech Instrument Co., Ltd.), an ultrasonic extractor (SB25-12DDT, Ningbo Xinzhi Biotechnology Co., Ltd.), and a freeze-drying instrument (SCIENTZ-10N, Ningbo Xinzhi Biotechnology Co., https://doi.org/10.1371/journal.pone.0318005.g001 PLOS ONE | https://doi.org/10.1371/journal.pone.0318005 January 27, 2025 3 / 20 Ltd.), an ultra-high performance liquid chromatography (UPLC) (ACQUITY, Waters, Milford), an ACQUITY UPLC1HSS T3 column (2.1×150 mm, 1.8 μm) (Waters, Milford), an Thermo Q Exactive (Thermo Fisher Scientific). ..
Microscopy:Article Title: Antioxidant and anti-inflammatory function of walnut green husk aqueous extract (WNGH-AE) on human hepatocellular carcinoma cells (HepG2) treated with t-BHP
Article Snippet: Finally, the IL-1β assay kit and TNF-α assay kit were sourced from Boster Bioengineering Co., Ltd. in Wuhan, China. .. The study utilized several instruments, including a double distilled deionized water system (laboratory purified water system, Shanghai Hitech Instrument Co., Ltd.), an electronic balance (PX224ENH, Germany OHAUS Instrument Co., Ltd.), a carbon dioxide incubator (HF90, Shanghai Heli Biopharmaceutical Holdings Co., Ltd.), an inverted biological microscope (BDS500, Chongqing Optec Instrument Co., Ltd.), an ELISA microplate analyzer (DNM-9602G, Beijing Perlove Co., Ltd.), a rotary evaporator (RE-2000A, Shanghai Hitech Instrument Co., Ltd.), an ultrasonic extractor (SB25-12DDT, Ningbo Xinzhi Biotechnology Co., Ltd.), and a freeze-drying instrument (SCIENTZ-10N, Ningbo Xinzhi Biotechnology Co., Ltd.), an ultra-high performance liquid chromatography (UPLC) (ACQUITY, Waters, Milford), an ACQUITY UPLC® HSS T3 column (2.1×150 mm, 1.8 μm) (Waters, Milford), an Thermo Q Exactive (Thermo Fisher Scientific). ..
Article Title: Antioxidant and anti-inflammatory function of walnut green husk aqueous extract (WNGH-AE) on human hepatocellular carcinoma cells (HepG2) treated with t-BHP.
Article Snippet: Finally, the IL-1β assay kit and TNF-α assay kit were sourced from Boster Bioengineering Co., Ltd. in Wuhan, China. .. The study utilized several instruments, including a double distilled deionized water system (laboratory purified water system, Shanghai Hitech Instrument Co., Ltd.), an electronic balance (PX224ENH, Germany OHAUS Instrument Co., Ltd.), a carbon dioxide incubator (HF90, Shanghai Heli Biopharmaceutical Holdings Co., Ltd.), an inverted biological microscope (BDS500, Chongqing Optec Instrument Co., Ltd.), an ELISA microplate analyzer (DNM-9602G, Beijing Perlove Co., Ltd.), a rotary evaporator (RE-2000A, Shanghai Hitech Instrument Co., Ltd.), an ultrasonic extractor (SB25-12DDT, Ningbo Xinzhi Biotechnology Co., Ltd.), and a freeze-drying instrument (SCIENTZ-10N, Ningbo Xinzhi Biotechnology Co., https://doi.org/10.1371/journal.pone.0318005.g001 PLOS ONE | https://doi.org/10.1371/journal.pone.0318005 January 27, 2025 3 / 20 Ltd.), an ultra-high performance liquid chromatography (UPLC) (ACQUITY, Waters, Milford), an ACQUITY UPLC1HSS T3 column (2.1×150 mm, 1.8 μm) (Waters, Milford), an Thermo Q Exactive (Thermo Fisher Scientific). ..
Enzyme-linked Immunosorbent Assay:Article Title: Antioxidant and anti-inflammatory function of walnut green husk aqueous extract (WNGH-AE) on human hepatocellular carcinoma cells (HepG2) treated with t-BHP
Article Snippet: Finally, the IL-1β assay kit and TNF-α assay kit were sourced from Boster Bioengineering Co., Ltd. in Wuhan, China. .. The study utilized several instruments, including a double distilled deionized water system (laboratory purified water system, Shanghai Hitech Instrument Co., Ltd.), an electronic balance (PX224ENH, Germany OHAUS Instrument Co., Ltd.), a carbon dioxide incubator (HF90, Shanghai Heli Biopharmaceutical Holdings Co., Ltd.), an inverted biological microscope (BDS500, Chongqing Optec Instrument Co., Ltd.), an ELISA microplate analyzer (DNM-9602G, Beijing Perlove Co., Ltd.), a rotary evaporator (RE-2000A, Shanghai Hitech Instrument Co., Ltd.), an ultrasonic extractor (SB25-12DDT, Ningbo Xinzhi Biotechnology Co., Ltd.), and a freeze-drying instrument (SCIENTZ-10N, Ningbo Xinzhi Biotechnology Co., Ltd.), an ultra-high performance liquid chromatography (UPLC) (ACQUITY, Waters, Milford), an ACQUITY UPLC® HSS T3 column (2.1×150 mm, 1.8 μm) (Waters, Milford), an Thermo Q Exactive (Thermo Fisher Scientific). ..
Article Title: Antioxidant and anti-inflammatory function of walnut green husk aqueous extract (WNGH-AE) on human hepatocellular carcinoma cells (HepG2) treated with t-BHP.
Article Snippet: Finally, the IL-1β assay kit and TNF-α assay kit were sourced from Boster Bioengineering Co., Ltd. in Wuhan, China. .. The study utilized several instruments, including a double distilled deionized water system (laboratory purified water system, Shanghai Hitech Instrument Co., Ltd.), an electronic balance (PX224ENH, Germany OHAUS Instrument Co., Ltd.), a carbon dioxide incubator (HF90, Shanghai Heli Biopharmaceutical Holdings Co., Ltd.), an inverted biological microscope (BDS500, Chongqing Optec Instrument Co., Ltd.), an ELISA microplate analyzer (DNM-9602G, Beijing Perlove Co., Ltd.), a rotary evaporator (RE-2000A, Shanghai Hitech Instrument Co., Ltd.), an ultrasonic extractor (SB25-12DDT, Ningbo Xinzhi Biotechnology Co., Ltd.), and a freeze-drying instrument (SCIENTZ-10N, Ningbo Xinzhi Biotechnology Co., https://doi.org/10.1371/journal.pone.0318005.g001 PLOS ONE | https://doi.org/10.1371/journal.pone.0318005 January 27, 2025 3 / 20 Ltd.), an ultra-high performance liquid chromatography (UPLC) (ACQUITY, Waters, Milford), an ACQUITY UPLC1HSS T3 column (2.1×150 mm, 1.8 μm) (Waters, Milford), an Thermo Q Exactive (Thermo Fisher Scientific). ..
Liquid Chromatography:Article Title: Antioxidant and anti-inflammatory function of walnut green husk aqueous extract (WNGH-AE) on human hepatocellular carcinoma cells (HepG2) treated with t-BHP
Article Snippet: Finally, the IL-1β assay kit and TNF-α assay kit were sourced from Boster Bioengineering Co., Ltd. in Wuhan, China. .. The study utilized several instruments, including a double distilled deionized water system (laboratory purified water system, Shanghai Hitech Instrument Co., Ltd.), an electronic balance (PX224ENH, Germany OHAUS Instrument Co., Ltd.), a carbon dioxide incubator (HF90, Shanghai Heli Biopharmaceutical Holdings Co., Ltd.), an inverted biological microscope (BDS500, Chongqing Optec Instrument Co., Ltd.), an ELISA microplate analyzer (DNM-9602G, Beijing Perlove Co., Ltd.), a rotary evaporator (RE-2000A, Shanghai Hitech Instrument Co., Ltd.), an ultrasonic extractor (SB25-12DDT, Ningbo Xinzhi Biotechnology Co., Ltd.), and a freeze-drying instrument (SCIENTZ-10N, Ningbo Xinzhi Biotechnology Co., Ltd.), an ultra-high performance liquid chromatography (UPLC) (ACQUITY, Waters, Milford), an ACQUITY UPLC® HSS T3 column (2.1×150 mm, 1.8 μm) (Waters, Milford), an Thermo Q Exactive (Thermo Fisher Scientific). ..
Article Title: Antioxidant and anti-inflammatory function of walnut green husk aqueous extract (WNGH-AE) on human hepatocellular carcinoma cells (HepG2) treated with t-BHP.
Article Snippet: Finally, the IL-1β assay kit and TNF-α assay kit were sourced from Boster Bioengineering Co., Ltd. in Wuhan, China. .. The study utilized several instruments, including a double distilled deionized water system (laboratory purified water system, Shanghai Hitech Instrument Co., Ltd.), an electronic balance (PX224ENH, Germany OHAUS Instrument Co., Ltd.), a carbon dioxide incubator (HF90, Shanghai Heli Biopharmaceutical Holdings Co., Ltd.), an inverted biological microscope (BDS500, Chongqing Optec Instrument Co., Ltd.), an ELISA microplate analyzer (DNM-9602G, Beijing Perlove Co., Ltd.), a rotary evaporator (RE-2000A, Shanghai Hitech Instrument Co., Ltd.), an ultrasonic extractor (SB25-12DDT, Ningbo Xinzhi Biotechnology Co., Ltd.), and a freeze-drying instrument (SCIENTZ-10N, Ningbo Xinzhi Biotechnology Co., https://doi.org/10.1371/journal.pone.0318005.g001 PLOS ONE | https://doi.org/10.1371/journal.pone.0318005 January 27, 2025 3 / 20 Ltd.), an ultra-high performance liquid chromatography (UPLC) (ACQUITY, Waters, Milford), an ACQUITY UPLC1HSS T3 column (2.1×150 mm, 1.8 μm) (Waters, Milford), an Thermo Q Exactive (Thermo Fisher Scientific). ..
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